Down regulation of GGTβ decreases dendrite growth and branching of PCs. A) HEK293 cells were co-transfected with Myc-GGTα and HA-GGTβ, together with pSUPER-GGTβ-siRNA or pSUPER. Cell lysates were subjected to IB with HA, Myc, GFP, or β-actin antibodies. B) Cultured cerebellar slices were transfected with pSUPER or pSUPER-GGTβ-siRNA, followed by staining with antibodies against GGTβ and Calbindin. Note the decrease of GGTβ signals in GGTβ-siRNA -transfected PCs. Scale bar = 20 μm. C) Cultured cerebellar slices at DIV2 were transfected with pSUPER-GGTβ-siRNA or pSUPER, together with pCAG-EYFP plasmid (3:1) to mark transfected cells. Shown are representative images at DIV5. Scale bar = 20 μm. D) Quantification for the number of crossings between dendrites and circles with a radius of indicated distance. E) Quantification for total dendritic length in indicated fields. Data shown are presented as means ± SEM (n = 17 for control, n = 23 for GGTβ-siRNA). *P < 0.05, Student's t test. Scale bar is 20 μm.