Figure 5
From: Geranylgeranyltransferase I is essential for dendritic development of cerebellar Purkinje cells
Rescue effect of siRNA-resistant form of GGTβ. A) Representative images of Purkinje cells after transfection with pSUPER (control), pSUPER-GGTβ-siRNA, or GGTβ-siRNA together with HA-GGTβ or HA-GGTβRes. B) Quantification for the number of crossings. C) Quantification for total dendritic length between neighboring circles. D) Number of crossings at the circle with radius of 75 μm was quantitatively analyzed. E, F) Total dendritic length between neighboring circles at the interval of 25 μm (between 50 and 75 μm or between 75 and 100 μm). Data are shown as means ± SEM (n = 12 for control; n = 17 for GGT-siRNA; n = 15 for GGT-siRNA plus HA-GGTβ; n = 19 for GGT-siRNA plus HA-GGTβRes). N.S. P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001. Student's t test. Scale bar = 20 μm.