Skip to main content
Figure 1 | Molecular Brain

Figure 1

From: Dopamine D1 receptor-mediated NMDA receptor insertion depends on Fyn but not Src kinase pathway in prefrontal cortical neurons

Figure 1

Expression of D1 and NMDA receptors in cultured PFC neurons. (A) Localization of D1 receptor and NR2B in cultured PFC neurons. PFC neurons in dissociated culture at 14 DIV were labeled for endogenous D1 and NR2B receptors with double immunofluorescent staining. Lower panel, images at higher magnification showing the colocalization of D1 and NR2B on the dendritic shafts and spines. Scale bars = 10 μm. (B) Analysis by Western blotting shows the protein expression of D1 receptor and NMDA receptor subunits in neuronal lysates. Crude synaptosome (20 μg of protein) from adult rat cortex (lane 1) and 14 DIV cultured PFC neurons (lane 2) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and probed for NR1, NR2A, NR2B, D1, PSD95, and adaptin. Both adult rat cortex crude synptosome and cultured PFC neurons exhibited similar expressions of NMDA and D1 receptors, as well as synaptic protein, suggesting the validity of the experiments in primary cultured neurons.

Back to article page

Molecular Brain

ISSN: 1756-6606