Figure 6

Src knockdown has no effect on the D1 receptor-mediated localization and clustering of NR2B. (A) Immunostaining revealed that NR2B expression was significantly increased by SKF-81297 (10 μM) treatment in Src siRNA-treated PFC neurons, compared with the control group. Scale bar = 10 μm. (B) Quantification of total NR2B immunofluorescence staining revealed that SKF treatment increased total NR2B puncta number and fluorescence intensity in dendrites even after Src siRNA transfection, compared with control. Results are presented as the mean number of total NR2B puncta and mean intensity of fluorescence (**p < 0.01). Statistical analysis was performed by t test and the data represent mean ± SEM. (C) Similarly, total protein or NR2B Tyr1472 phosphorylation levels of NR2B determined by Western blotting after Src siRNA transfection remained to be significantly altered by SKF-81297 treatment in cultured PFC neurons (* p < 0.05). Control NR2B protein level or NR2B Tyr1472 phosphorylation level was set to 100% after being normalized by loading control tubulin.