Figure 2
From: Transactivation of PDGFRβ by dopamine D4 receptor does not require PDGFRβ dimerization
PDGFRβ siRNAs inhibit dopamine-mediated ERK1/2 activation. (A) 100 nM of double-stranded PDGFRβ interference RNA (PDGFRβ silencer 1) or (B) 30 nM of double-stranded PDGFRβ RNA (PDGFRβ silencer 2) was transfected into the CHO/DRD4 cells. Cells were harvested at 72 h post transfection and PDGFRβ expression was determined. For phospho-ERK1/2 expression (Thr202/Tyr204), cells were serum-starved overnight (beginning at 48 h post-transfection) and subsequently treated with dopamine (1 βM) or PDGF-BB (10 ng/ml).