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Figure 5 | Molecular Brain

Figure 5

From: Roles of KChIP1 in the regulation of GABA-mediated transmission and behavioral anxiety

Figure 5

KChIP1 inhibits potassium currents in cultured hippocampal neurons. a: Co-immunoprecipitation of Kv4.3 and KChIP1-EGFP. HEK293 cell lysates co-expressing Flag-tagged Kv4.3 and KChIP1-EGFP were immunoprecipitated with an anti-Flag antibody (left panel; IP: Kv4.3) or an anti-EGFP antibody (right panel; IP: EGFP) followed by immunoblotting with anti-EGFP (left panel; IB: EGFP) or anti-Flag (right panel; IB: Kv4.3), respectively. b: Potentiation of Kv4.3 current density by Kv4.3-EGFP in Xenopus oocytes. Kv4.3 was co-expressed with either KChIP1-EGFP fusion protein, KChIP1, or EGFP in Xenopus oocytes. KChIP1-EGFP and KChIP1 but not EGFP significantly increased Kv4.3 current density (*P < 0.001). c, d: KChIP1-EGFP modulates inactivation of Kv4.3 in Xenopus oocytes. Kv4.3 was co-expressed with either KChIP1-EGFP fusion protein (black line), KChIP1 (red line), or EGFP (green line) in Xenopus oocytes. Expression of KChIP1-EGFP or KChIP1 enhanced inactivation of Kv4.3 from both the open state (c) and the closed state (d) compared with EGFP alone.

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