Figure 1

Enhanced synaptic potentiation in ACC neurons of CaMKIV transgenic mice mediated by transcription activity. A: The paired training inducing LTP in ACC neurons of wild-type mice (n = 8/5 mice). Partial inhibition of LTP by 40 μM actinomycin-D in ACC neurons of wild-type mice (n = 9/5 mice). B: Enhanced LTP in ACC neurons of CaMKIV transgenic (TG) mice (n = 8/5 mice). Marked inhibition of LTP by actinomycin-D in ACC neurons of CaMKIV TG mice (n = 7/5 mice). A-B: The insets showing averages of five EPSCs at baseline responses (pre) and at 25-30 min (30 min post) after the paired training (arrow); the dashed lines indicating the mean basal synaptic responses. C: Statistical results showing the significant inhibition of LTP by actinomycin-D at 15-20 and 25-30 min after induction of LTP in ACC neurons of wild-type mice. The dashed line indicating the mean basal synaptic responses. *P < 0.05 compared to control. D: Statistical results showing the significant inhibition of LTP by actinomycin-D at 0-5, 15-20 and 25-30 min after induction of LTP in ACC neurons of CaMKIV TG mice. The dashed line indicating the mean basal synaptic responses. **P < 0.01 compared to control. E: Actinomycin-D (Act-D) sensitive synaptic potentiation in ACC neurons of CaMKIV TG mice is larger than that of wild-type mice. F: Statistical results showing that Act-D sensitive synaptic potentiation in ACC neurons of CaMKIV TG mice is significantly larger than that of wild-type mice at 0-5, 15-20 and 25-30 min after induction of LTP. *P < 0.05, **P < 0.01 compared to wild-type mice.