Overexpression of CaMKIV in the ACC neurons of the CaMKIV transgenic mice. A: CaMKIV immunoreactive (IR) neurons are mainly distributed in layer II/III of ACC in the wild-type (WT) mice. B: A larger number of CaMKIV-IR neurons are observed in layers II/III and V/VI of ACC in the CaMKIV transgenic (TG) mice. C-D: (C) and (D) showing enlarged images from rectangle areas in (A) and (B), respectively. Overexpression of CaMKIV immunoreactivity in the nuclei was observed in CaMKIV TG mice. E: Summary data of CaMKIV in ACC from the WT and CaMKIV TG mice. Bars equal to 50 μm in (A) and (B) and 10 μm in (C) and (D). ***, P < 0.001.