Figure 3

Reduction of pairing protocol-induced long-term depression in the hippocampus in TgDREAM mice. (A) Diagram showing the paring protocol for LTP induction. The holding potential is +30 mV and 80 presynaptic stimulations at 2 Hz were applied. (B) LTP induced in hippocampal CA1 pyramidal neurons in wild-type mice (filled circles, n = 6) and TgDREAM mice (open circles, n = 6) by pairing protocol. In the present and following figures, the insets show averaged EPSC at 5 and 25 min after the pairing procedure (arrow). The dashed line indicates the mean basal synaptic response. (C) Pooled data show no significant difference (n.s.) of LTP in wild-type and TgDREAM mice. (D) Diagram showing the pairing protocol for LTD induction. The holding potential is -45 mV and 300 presynaptic stimulations at 1 Hz were applied. (E) LTD was stably induced by pairing protocol in wild-type mice (filled circles, n = 8), but completely abolished in TgDREAM mice (open circles, n = 8). (F) Pooled data show a significant difference in LTD between wild-type and TgDREAM mice. ** P < 0.01.