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Figure 6 | Molecular Brain

Figure 6

From: DREAM (Downstream Regulatory Element Antagonist Modulator) contributes to synaptic depression and contextual fear memory

Figure 6

DREAM interacts with PSD95 protein in vivo. (A) Co-immunoprecipitation of DREAM with PSD-95 in mouse hippocampal extracts from wild type mice. DREAM antibody immunoprecipitated DREAM bound to PSD-95 (left) while the PSD-95 antibody immunoprecipitated PSD-95 along with DREAM (right). Neither PSD-95 nor DREAM was immunoprecipitated using a control IgG. (B) DREAM antibody immunoprecipitated DREAM, but no NMDA receptor subunits (NR1, NR2A or NR2B) was detected in the immunoprecipitation. (C) PSD-95 protein was immunoprecipitated from mouse hippocampal extracts by a specific DREAM antibody. Increasing Ca2+ concentrations (0.1 and 0.25 mM) prevent this interaction. Absence of Ca2+ (0) corresponds to 2 mM EDTA.

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Molecular Brain

ISSN: 1756-6606