The novel Sox10- Venus transgenic mouse strain. The Sox10- Venus BAC transgenic mouse strain was generated to investigate the Sox10 expression profile in vivo. (A) Venus could be detected in NC lineage tissues, including the DRG and trigeminal ganglia, in E11.5 d mice. Sox10+ cells that were not NC-derived, including cells in the otic vesicle, could also be visualized with Venus. (B) High-intensity fluorescence enabled us to observe a migration stream at the single-cell level, from outside the embryo. (C) At E15.5 d, deep-tissue Venus fluorescence decreased. At this age, migrating Venus+ cells and the network formation of Venus+ cells in the superficial skin layer could be traced. (D) The posterior branches of the spinal nerve were clearly visualized with Venus+ Schwann cells, along with peripheral nerve fibers. (E) In the hind limb, the peripheral nerve and vascular network formations were clearly outlined. (F) The peripheral neural network mainly derived from cranial nerve VII could be visualized with three-dimensional reconstruction with an Olympus MVX-CSU microscope. (G) Time-lapse imaging of the front facial area of embryonic Sox10- Venus mice clearly showed individual Venus+ cell movement (also shown in movies in Additional Files 2 and 3). (B) and (D, E) are high-magnification images of the indicated areas in Figures (A) and (C), respectively. Cranial nerves V, trigeminal nerve; VII, facial nerve; VIII, vestibulocochlear nerve, Scale bars; (A-F) 1.0 mm.