Figure 4

Effects of behavioral stress on phospho-eIF2α levels in the hippocampus and cerebral cortex of 5XFAD mice. Male and female mice were exposed to restraint stress for 6 h per day during 5 consecutive days and were sacrificed for analysis 24 h after the last stress treatment. Representative immunoblots of phosphorylated eIF2α (p-eIF2α) and total eIF2α for hippocampal homogenates of female 5XFAD mice with and without exposure to stress are shown along with those of non-stressed wild-type controls. Intensities of p-eIF2α-immunoreactive bands were quantified by phosphorimaging and were expressed as percentage of wild-type controls (n = 3-9 mice per group). Note that p-eIF2α levels, but not total eIF2α levels, are increased specifically in the hippocampus of stressed female 5XFAD mice as compared with non-stressed wild-type controls (^#p < 0.05) and non-stressed 5XFAD mice (*p < 0.05). All data are presented as mean ± SEM.