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Figure 6 | Molecular Brain

Figure 6

From: Nigrostriatal neuronal death following chronic dichlorvos exposure: crosstalk between mitochondrial impairments, α synuclein aggregation, oxidative damage and behavioral changes

Figure 6

Nigrostriatal dopaminergic degeneration. (A) Representative image show TH expression in SN area of dichlorvos treated rat and control littermate. (B) Bar graph depicts TH-immunoreactive, Cell counts in the SN of dichlorvos rat and control littermate. Values are mean ± S.D. (N = 6),** p< 0.01 significantly different from control group. (C). Semi quantitative RT-PCR analysis of TH from substantia nigra and corpus striatum of rat brain. (D) Densitometric analysis of TH/GAPDH. (E). The proteins separated on 10% SDS-PAGE were subjected to immunoblotting using anti-TH antibody. (F) Densitometric analysis blot. TH/β actin. (G) Effect of chronic dichlorvos exposure on DBH mRNA expression in SN and CS by RT-PCR (Agarose gel electrophoresis).(H) Densitometric analysis of DBH/GAPDH. (I). Effect of chronic dichlorvos exposure on DBH quantitative protein expression in substantia nigra and corpus striatum of rat brain. The proteins separated on 12% SDS-PAGE were subjected to immunoblotting using anti-DBH. (J) Densitometric analysis blot DBH/β actin. Values are mean SD of 4 animals in each group, *p < 0.05 significantly different from control group. Lanes 1,3: Control; Lanes 2,4: Dichlorvos treated; CSN: Control substantia nigra; DSN: Dichlorvos substantia nigra; CCS: Control corpus striatum; DCS: Dichlorvos corpus striatum.

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