Table 1 List of antibodies used in this study.
From: MATH5 controls the acquisition of multiple retinal cell fates
Antibodies | Sources | Working dilution | Retinal cell markers | References |
|---|---|---|---|---|
anti-BHLHB5 | Santa Cruz Biotech. (Santa Cruz, CA) | 1:1,000 | displaced amacrine, GABAergic amacrine, and OFF-cone bipolar cells | [16] |
anti-BrdU | DSHB (Univ. of Iowa) | 1:50 | cell cycle, S-phase cells | [16] |
anti-BRN3A | Chemicon Intl. (Temecula, CA) | 1:400 | retinal glanglion cells | [32] |
anti-BRN3B | Santa Cruz Biotech. (Santa Cruz, CA) | 1:2,000 | retinal ganglion cells | [32] |
anti-calbindin 28K | Sigma (St. Louis, MO) | 1:5,000 | horizontal cells, some amacrine cells | [33] |
anti-calretinin | Calbiochem (San Diego, CA) | 1:2,000 | ganglion and amacrine cells of cholinergic, non-AII and displaced subtypes | |
anti-activated caspase-3 | R&D Systems (Minneapolis, MN) | 1:200 | apoptotic cells | [36] |
anti-CHX10 | Exalpha (Watertown, MA) | 1:200 | progenitors, bipolar cells | [37] |
anti-GAD65 | BD Biosciences (San Jose, CA) | 1:200 | GABAergic amacrine cells | [38] |
anti-Goα | Upstate (Lake Placid, NY) | 1:200 | ON-bipolar cells | [33] |
anti-GFP | MBL (Woaburn, MA) or Abcam, (Cambridge, MA) | 1:1,000 | green fluoresence protein | [36] |
anti-lacZ | DSHB, Univ. of Iowa or Chemicon Intl. (Temecula, CA) | 1:500 | ß-galactosidase | [36] |
anti-ISL1/2 | DSHB (Univ. of Iowa) | 1:400 | retinal ganglion, ON-bipolar, and cholinergic amacrine cells | |
Anti-Ki67 | BD Pharmingen (San Jose, CA) | 1:200 | cell cycle, all phases | [42] |
anti-NEUROD1 | Santa Cruz Biotech. (Santa Cruz, CA) | 1:500 | cone and amacrine cells | [15] |
anti-NRL | Chemicon Intl. (Temecula, CA) | 1:1,000 | rod photoreceptor cells | [43] |
anti-p27kip1 | BD Biosciences (San Jose, CA) | 1:100 | Muller cells | [44] |
anti-phosphorylated histone 3 | Santa Cruz Biotech. (Santa Cruz, CA) | 1:400 | cell cycle, M-phase | [16] |
anti-PAX6 | DSHB (Univ. of Iowa) | 1:200 | progenitors, pan-ganglion, and pan-amacrince cells | |
anti-PKCα | Sigma (St. Louis, MO) | 1:5,000 | rod bipolar cells | [33] |
anti-PROX1 | Covance (Berkeley, CA) | 1:1,000 | progenitors, bipolar, horizintal, AII and displaced amacrince cells | [48] |
anti-recoverin | Chemicon Intl. (Temecula, CA) | 1:200 | Type 2 OFF-cone bipolar, photoreceptor cells | |
anti-rhodopsin | Chemicon Intl. (Temecula, CA) | 1:200 | Rod photoreceptor cells | [50] |
anti-RXRγ | Santa Cruz Biotech. (Santa Cruz, CA) | 1:200 | Cone photoreceptor cells | [51] |
anti-VSX1 | Gift from R.L. Chow (Univ. of Victoria, Canada) | 1:100 | OFF-cone bipolar cells |