Figure 4
From: NMDAR2B tyrosine phosphorylation regulates anxiety-like behavior and CRF expression in the amygdala
De-phosphorylation of Tyr-1472 and up-regulation of CRF expression after the EPM test and by NMDA-receptor stimulation. (A) The level of Tyr-1472 phosphorylation in the amygdala of WT mice was decreased by the EPM test (control, n = 6; EPM test, n = 4; p < 0.05, Student's t-test). A representative blot is shown in the upper panel. (B) Real-time PCR analyses showed that CRF expression in the amygdala of WT mice was increased after the EPM test (control, n = 6; EPM test, n = 4; p < 0.05, Student's t-test). (C) Real-time PCR analyses showed that NMDA stimulation (100 μM NMDA for 7 min) increased CRF expression in the amygdalae of WT mice (control, n = 6; NMDA stimulation, n = 6; p < 0.05, Student's t-test) but not in GluN2B-YF mice (control, n = 6; NMDA stimulation, n = 6; p > 0.2, Student's t-test). (D) The same NMDA stimulation also induced de-phosphorylation of Tyr-1472 in the amygdalae of WT mice (control, n = 5; NMDA stimulation, n = 5; p < 0.05, Student's t-test). A representative blot is shown in the upper panel. The asterisk indicates significant genotype differences. n.s., not significant.