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Figure 3 | Molecular Brain

Figure 3

From: The modulation of TRPM7 currents by nafamostat mesilate depends directly upon extracellular concentrations of divalent cations

Figure 3

NM attenuates low-divalent-cation-induced Ca2+ influx and action potentials in cultured hippocampal neurons. A, calcium image; representative changes in ratio images (top) and 340/380 nm ratio values (bottom) by lowering Ca2+ from 2 mM to 0.5 mM and Mg2+ from 1 mM to 0 mM. NM (50 μM) was co-applied with low divalent cations. B, summarized data shows the reduction of low divalent-cation-induced increase of 340/380 nm ratio by NM, **p < 0.01. n = 11. B. NM (200 μM) reduced the frequency of action potentials in on-cell loose patch induced by lowering divalent cations. Action potentials were induced by 0.5 mM Ca2+ and 0.5 mM Mg2+. Left, trace examples; right, statistic analysis. Black circle shows the mean of firing frequency. Firing frequency was significantly (p < 0.05) reduced from 8.5 ± 2.7 Hz (control) to 6.8 ± 2.9 Hz (adding NM) and back to 8.0 ± 2.4 Hz after washing out NM, n = 16.

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