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Figure 3 | Molecular Brain

Figure 3

From: PICK1 regulates the trafficking of ASIC1a and acidotoxicity in a BAR domain lipid binding-dependent manner

Figure 3

BAR domain mutant PICK1 2K-E still interacts with ASIC1a. HEK293T cells were transfected with GFP-ASIC1a together with myc-tagged wild-type PICK1, PICK1 2K-E, PICK1 KD-AA or the vector control, as indicated on the top. Cell lysates were immunoprecipitated with an anti-myc antibody and immunoblotted with either a GFP or a PICK1 antibody as indicated. The BAR domain mutant PICK1 2K-E co-immunoprecipitated with ASIC1a similar to wild-type PICK1 but the PDZ mutant PICK1 KD-AA could not co-immunoprecipitate with ASIC1a.

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Molecular Brain

ISSN: 1756-6606