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Figure 5 | Molecular Brain

Figure 5

From: Pyk2 uncouples metabotropic glutamate receptor G protein signaling but facilitates ERK1/2 activation

Figure 5

ERK1/2 phosphorylation in mouse cortical neurons is mGluR1-dependent. A) Representative immunoblot of the time course for ERK1/2 phosphorylation in primary mouse cortical neurons treated with 50 μM DHPG. Bar graph shows the denstiometric analysis of the time course of DHPG-stimulated ERK1/2 phosphorylation in cortical neurons. The data represents the mean ± SD of four independent experiments. * P < 0.05 versus untreated cells. B) Representative immunoblot showing the effect of treating primary mouse cortical neurons with and without 50 μM DHPG for 5 min following pretreatment either with or without the mGluR1-selective antagonist LY367385 (100 μM) on ERK1/2 phosphorylation. Bar graph shows the mean ± SD of five independent experiments. C) Representative immunoblot showing the effect of treating primary mouse cortical neurons with and without 50 μM DHPG for 5 min following pretreatment either with or without the mGluR5-selective antagonist MPEP (10 μM) on ERK1/2 phosphorylation. Bar graph shows the mean ± SD of four independent experiments. * P < 0.05 versus untreated mGluR1a expressing cells.

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