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Figure 2 | Molecular Brain

Figure 2

From: The physiological roles of vesicular GABA transporter during embryonic development: a study using knockout mice

Figure 2

Neurotransmitter contents and expression levels of GAD65 and GAD67. (A) Neurotransmitter contents of E18.5 mouse forebrain. VGAT-/- mice showed significantly higher levels of GABA and glycine than VGAT+/+ mice, but not glutamate. Values represent means ± SD (*P < 0.05; Student's t-test, n = 5-13 per group). (B) Western blot analysis. The expression level of GAD65 and GAD67 in whole brain homogenates was not significantly different between VGAT+/+ (+/+) and VGAT-/- (-/-) mice. Equal amounts of protein were loaded and probed with an antibody that recognizes both GAD65 and GAD67. For the statistical comparison, the same blot was probed with anti-β-actin antibody as an internal control and measurements for GAD65 and GAD67 bands were normalized to the β-actin bands (GAD65: VGAT+/+ 100 ± 23%; VGAT-/- 111 ± 20%, n = 4, P = 0.71) (GAD67: VGAT+/+ 100 ± 18%; VGAT-/- 93 ± 17%, n = 4, P = 0.80).

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