Figure 4

Histological analysis of VGAT^-/- mice. Histological analysis (hematoxylin and eosin staining) of trapezius muscle (A, B), sagittal sections (C, D), liver (E, F) and lung (G, H) from E18.5 VGAT^-/- mice (B, D, F, H) and control mice (A, C, E, G). (A, B) The trapezius muscle (bounded partly by white dashed lines) of VGAT^-/- mouse (B) was thinner than the control mouse (A). Scale bar: 200 μm. (C, D) The VGAT^-/- ribs (arrow in D) in the lower part were depressed, and their position was inside compared to the control ribs (arrow in C). H, heart; L, liver. (E, F) Red blood cell congestion was characteristic of VGAT^-/- liver, but not control liver. Scale bar: 200 μm. (G, H) The VGAT^-/- lung contained much less alveolar space than the control lung. Scale bar: 500 μm.