Inhibition of protein translation in spinal neurons by C.mycin -induced GyrB-PKR dimerization. (A) Expression of the pd1-EGFP in Xenopus spinal neurons. Bright field, fluorescent, and merged images (left, middle, right, respectively) show a spinal neuron and muscle cells expressing pd1-EGFP. N: neuron; M: muscle cell. Bar, 10 μm. (B) Quantification of fluorescence change on neurons. Pd1-EGFP was expressed either alone or together with GyrB-PKR in Xenopus spinal neurons, using embryo injection techniques. C.mycin was applied to the culture dish at time "0", and pd1-EGFP fluorescence was monitored over time. The number of experiments performed was indicated in the legend.