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Figure 3 | Molecular Brain

Figure 3

From: Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation

Figure 3

Normal acute synaptic potentiation by NT-3 in GyrB-PKR activated neuromuscular synapses. (A) Sample recordings showing acute synaptic potentiation by NT-3. NT-3 (50 ng/ml) was applied directly to 1-day-old nerve-muscle co-culture. The frequency of spontaneous synaptic currents (SSCs) was used to monitor changes in synaptic efficacy. (B) A sample image showing neuromuscular synapses in which muscle cells are innervated by a GFP expressing spinal neuron. N: neuron axon (neuron body is out of this image); M: muscle cell. Bar, 10 μm. (C) Summary of acute effect of NT-3 on synapses without GyrB-PKR expression, and synapses with presynaptic or postsynaptic expression of GyrB-PKR. Each data point represents SSCs frequency (averaged from 30 min of recording) from a single synapse before C.mycin and after NT-3 application. Note that the application of coumermycin did not affect the baseline of recording. The number associated with each column represents the number of cells analyzed. Data are presented as the mean ± the SEM.

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