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Figure 6 | Molecular Brain

Figure 6

From: si-RNA inhibition of brain insulin or insulin-like growth factor receptors causes developmental cerebellar abnormalities: relevance to fetal alcohol spectrum disorder

Figure 6

Effects of ICV injection of si-RNA targeting insulin or IGF receptors on indices of neuronal structure and function. Long Evans rat pups were administered ICV injections of si-Scr, si-InR, si-IGF-1R, or si-IGF-2R on P2. Cerebella harvested on P24 were used to measure (A) Tau, (B) phospho-Tau; pTau), (C) choline acetyltransferase; ChAT (D) acetylcholinesterase; AChE, (E) β-Actin, and (F) GAPDH immunoreactivity by ELISA with results normalized to protein content within the wells. Immunoreactivity was detected with horseradish peroxidase conjugated secondary antibody and the Amplex Red fluorophore. Fluorescence was measured in a Spectramax M5 microplate reader (Ex 530 nm/Em 590 nm). Box plots depict means, 95% confidence intervals, and range for each group. Inter-group statistical comparisons were made using the Kruskal-Wallis one-way ANOVA and the Dunn's multiple comparisons test. Significant P-values are indicated within the panels.

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Molecular Brain

ISSN: 1756-6606