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Figure 1 | Molecular Brain

Figure 1

From: Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells

Figure 1

The 184-kb Msi1- reporter expression corresponds to endogenous Msi1 expressed in NS/PCs in vivo. (A) The original BAC (RP24-132L16) and the position of Msi1 are shown. There was a 22-kb region of Msi1 exons and introns between the 133-kb 5' upstream and 29-kb 3' downstream regions of Msi1. The translation initiation codon (ATG) was replaced with ffLuc to produce Msi1-ffLuc. (B) GFP in the E12.5 transgenic mouse containing Msi1-ffLuc, was expressed in the central nervous system and eye (upper panel: bright-field image, lower panel: fluorescent image). (C) Immunohistochemical analysis in the E14.5 Tg mouse cortex. Msi1, Group B1 SOX [SOX1/(2)/3], and Nestin expression in undifferentiated cells in the ventricular zone co-localized with GFP expression (Scale bar: 50 μm). The images shown in the small boxes in the right column panels are magnified and shown in the left corner boxes (Scale bar in magnified image: 10 μm). GFP was also present in the ventricular zone of the midbrain (D), pontine area (E), and eye (F). An arrow shows expression of GFP in retinal ganglion cell layer and an arrowhead shows expression of GFP in lens. AQ: aqueduct of midbrain, V4: fourth ventricle. Scale bar: 100 μm.

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Molecular Brain

ISSN: 1756-6606