Figure 1

A loss of UNC-7 function does not affect NMJ synaptic transmission. (A) Representative traces of spontaneous mPSC (left) and electrically evoked EPSC (right) in wild type (wt) and unc-7 mutant animals (upper panels). Lower panels show the quantification of spontaneous mPSC amplitude and frequency, and electrically evoked EPSC amplitude in wild type (wt) and unc-7 animals. (B, C) GABA and acetylcholine receptors in unc-7 mutants were functional. Pressure ejection of GABA (0.1 mM) (B) or acetylcholine, Ach (0.1 mM) (C) evoked similar EPSCs in unc-7 mutant as in wild type animals. Detection of cholinergic (D) and GABAergic (E) light-evoked synaptic responses (upper traces) in animals expressing ChR2 in the corresponding motoneurons (zxIs6 and zxIs3, respectively). Quantifications of amplitude from light-evoked responses in wild type and unc-7 animals are shown in the lower panels. Animals were subjected to trains of stimulations at 20 Hz (left panels) and 0.5 Hz (right panels).