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Figure 2 | Molecular Brain

Figure 2

From: mRNA binding protein staufen 1-dependent regulation of pyramidal cell spine morphology via NMDA receptor-mediated synaptic plasticity

Figure 2

Impairment of FSK-induced L-LTP after Stau1 knockdown during high Mg2+ treatment. (A) Potentiation of fEPSP slope induced by FSK application (50 μM, 15 min) in cultured slices maintained in medium containing high Mg2+ for 48 hours after siRNA-CTL or siRNA-STAU1 transfection. For electrophysiological experiments, slices were tested in conditions with normal NMDA receptor function. Corresponding field potentials before (black line) and after (gray line) FSK application are shown at right. (B) Summary bar graph showing changes in fEPSPs slope 200 min post-FSK application. Significant L-LTP was present in slices transfected with siRNA-CTL but absent in slices transfected with siRNA-STAU1, indicating that Stau1 knockdown still prevents L-LTP after siRNA-STAU1 transfection in high Mg2+. *, P < 0.05, t-test. Error bars represent s.e.m. (C-D) Stau1 siRNA transfection did not affect basal synaptic transmission (C, input-output function; D, paired-pulse facilitation ratio).

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