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Table 1 Spine changes induced by NMDA receptor blockade, high Mg2+ and Stau1 siRNA treatment.

From: mRNA binding protein staufen 1-dependent regulation of pyramidal cell spine morphology via NMDA receptor-mediated synaptic plasticity

 

siRNA-CTL

siRNA-STAU1

 

Normal medium

High Mg2+ (12 mM)

AP5

(100 μM)

Normal medium

High Mg2+ (12 mM)

AP5

(100 μM)

Spine density (spine/μm)

0.29 ± 0.02

0.21 ± 0.02*

0.34 ± 0.03

0.28 ± 0.02

0.19 ± 0.02

0.34 ± 0.03

Spine length (μm)

1.15 ± 0.02

1.3 ± 0.02*

1.3 ± 0.02*

1.29 ± 0.02§

1.35 ± 0.03

1.32 ± 0.03

Spine shape

(% of total)

      

Regular

49.75 ± 3.1

32.3 ± 2.7*

37.9 ± 2.2*

31.77 ± 2.2§

30.19 ± 3

36.8 ± 2.6

Elongated

40.92 ± 2.3

54.68 ± 2.3*

50.3 ± 2.3*

51.49 ± 2§

51.35 ± 2.7

53.1 ± 2.7

Filopodia

9.33 ± 1.5

13.02 ± 1.9

11.8 ± 1.6

16.74 ± 1.4

15.57 ± 3

10.2 ± 1.5

  1. - Data expressed as mean ± s.e.m from 16-27 neurons per group, 4-8 independent experiments per group; in total 3129 protrusions were analyzed from 101 neurons.
  2. - * indicates significant difference in siRNA-CTL relative to normal medium; P < 0.05, ANOVA.
  3. - § indicates significant difference in siRNA-STAU1 relative to siRNA-CTL in individual condition; P < 0.05, ANOVA.
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Molecular Brain

ISSN: 1756-6606