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Figure 5 | Molecular Brain

Figure 5

From: Long-term changes of spine dynamics and microglia after transient peripheral immune response triggered by LPS in vivo

Figure 5

LPS induced persistent activation of microglia. (A) Immunohistochemical analyses were performed with fixed cortical slices from control and LPS treated mice at different time points. Slices were stained either with anti-Iba1 antibody for microglia or anti-GFAP antibody for astrocytes. Projection images are shown. Scale bar in the upper column, 10 μm; bar in the lower column, 400 μm. (B) Temporal profile of enhanced Iba1 expression after LPS treatment. To evaluate expression of Iba1, the total intensity of fluorescence was measured and the averages were plotted as values normalized to the control (N: number of mice, n: number of slices). The intensity of fluorescence was significantly increased after two days of LPS treatment and this increasing trend was maintained until 28 days. (C) Late increase in the number of microglia after LPS treatment. The number of microglia was counted and the averages were plotted as values normalized to the control (N: number of mice, n: number of slices). The number of microglia was significantly increased only at 28 days after LPS treatment. **, p < 0.01, ***, p < 0.001.

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