Figure 6

Morphological changes of microglia after LPS treatment. (A) High magnification images of microglia in Iba1-GFP mice. Mice were either unperturbed or received LPS injection and were sacrificed for immunohistochemical analyses two, seven, and 28 days later. Scale bar, 10 μm. (B) Total lengths of microglial processes were measured and the averages are shown as lengths normalized to the control (N: number of mice, n: number of microglia). The shrinkage of processes was observed at two days but they recovered their original lengths seven days after LPS injection. GFP signals from the microglial processes were enhanced even at later time points (seven and 28 days), but this may be due to either real increase in process diameters or increases in the GFP protein content. ***, p < 0.001.