Figure 5From: Fetal alcohol exposure leads to abnormal olfactory bulb development and impaired odor discrimination in adult miceEffect of fetal alcohol exposure on olfactory bulb neurogenesis. (a) Postnatal OB neurogenesis was assessed using 3 methods: (i) in vitro neurosphere assay, (ii) in vivo labeling with BrdU, and (iii) in vivo labeling with LacZ using a transgenic reporter system. (b,c) FAE mice (n = 9) had fewer in vitro neural precursor cells and fewer small neurospheres in the SEZ compared to control mice (n = 9) between P4 and P14. (d) FAE mice had fewer BrdU+ cells in the granule cell layer of the OB compared to control mice P7 (control n = 9, FAE n = 8) and P21 (control n = 8, FAE n = 9). (e) There was no difference in BrdU+ cells between control (n = 8) and FAE mice (n = 8) at P60. (f) FAE reporter mice had fewer LacZ+ cells in the granule cell layer of the OB at P30 (control n = 8, FAE (10%) n = 6, FAE (15%) n = 9).Back to article page