Figure 1

NMDA and DHPG differentially regulate trafficking of SEP-GluA2 in cultured hippocampal neurons. A. Representative images of cultured hippocampal neurons infected with SEP-GluA2 prior to NMDA treatment (i), 5 min post addition of NMDA (ii) and 35 min post addition of NMDA (iii). B Representative images of cultured hippocampal neurons infected with SEP-GluA2 prior to DHPG treatment (i), 5 min post addition of DHPG (ii) and 35 min post addition of DHPG (iii). C. Representative images of cultured hippocampal neurons pre treated with 1 mM orthovanadate prior to DHPG treatment (i), 5 min post addition of DHPG (ii), and 35 min post addition of DHPG (iii). Example regions of interest (ROI) of the kind that were used for analysis are highlighted in red (dendritic shaft) and blue (puncta). Calibration represents 20 μm on the main images and 10 μm on insets.