Analysis of SEP-GluA2 fluorescence in ROIs in the dendritic shaft (red lines) and in punta (blue lines). A. Application of NMDA (black bar) results in a rapid and reversible reduction in average fluorescence in the dendritic shaft, followed by a later reduction in average fluorescence in puncta. B. Application of DHPG (black bar) results in a slow reduction in average dendritic shaft fluorescence but no change in average puncta fluorescence over the time period studied. C. The PTP inhibitor Ov applied continuously during the experiment (grey bar) prevents the reduction in shaft SEP-GluA2 fluorescence induced by DHPG (black bar).