Figure 3

Analysis of fluorescence from individual ROIs reveals heterogeneous responses to application of agonists. A. Application of NMDA (black bar) results in a rapid and reversible decrease in fluorescence in ROIs on the dendritic shaft; reflected in the majority of ROIs showing negative values at 10 min (i) but not at 40 mins (ii). B. Application of NMDA (black bar) results in a slow decrease in fluorescence in ROIs on puncta, reflected in many ROIs showing no change in fluorescence at 10 min (i), but decreases in fluorescence at 40 min (ii). C. Application of DHPG (black bar) results in slow decreases in fluorescence in ROIs on the dendritic shaft; reflected in the majority of ROIs showing no change at 10 min (i) but decreased fluorescence at 40 min (ii). D. Application of DHPG (black bar) results in variable changes in fluorescence in ROIs on puncta. Most ROIs show no change in fluorescence at 10 min (i), however at 40 min puncta show both decreases and increases in fluorescence (ii). DHPG applied in the presence of Ov results in a substantially smaller change in fluorescence in ROIs on either the dendritic shaft (E) or puncta (F) at 10 min (i) or at 40 min (ii).