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Figure 1 | Molecular Brain

Figure 1

From: Activation of protease activated receptor 1 increases the excitability of the dentate granule neurons of hippocampus

Figure 1

Immunohistochemistry reveals that PAR1 receptor is expressed in dentate granule cells. A, B, C) Image of dentate in a thin section from a C57Bl/6 mouse, immunostained with thrombin receptor polyclonal antibody (S-19, Santa Cruz). Red indicates DAPI staining (A) and green indicates PAR1 immunostaining (B). PAR1-immunoreactivity is clearly expressed in the dentate granule cell layer (C); scale bar: 50 μm. Data are representative of three different experiments from two different mice. D) Western blot of PAR1 with thrombin receptor polyclonal antibody (S-19, Santa Cruz) in cultured astrocytes transfected with scrambled shRNA or PAR1 shRNA. 66 kDa PAR1 and 50 kDa actin bands are presented. This experiment was performed three times from three different mice. E) Immunocytochemistry of PAR1 with thrombin receptor polyclonal antibody (S-19, Santa Cruz) in cultured astrocytes. Green indicates cultured astrocytes transfected with PAR1 shRNA. Magenta shows GFAP, a marker for all GFAP-positive astrocytes. Red represents PAR1 immunostaining. The arrow indicates a cultured astrocyte that expresses PAR1 shRNA, whereas the arrowhead indicates an astrocyte without expression of shRNA. Staining in cultured astrocyte was performed in permeabilized conditions. This experiment was performed three times from three different mice. Scale bar is 50 μm. F) Immunostaining with PAR1 antibody (WEDE15) in the dentate gyrus using DAB staining; scale bar is 150 μm. G) Western blot of PAR1 with PAR1 monoclonal antibody (WEDE15, Immunotech) in mouse whole brain homogenates. A single band corresponding to the predicted molecular weight of PAR1 (66 kDa) was observed.

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