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Figure 5 | Molecular Brain

Figure 5

From: Activation of protease activated receptor 1 increases the excitability of the dentate granule neurons of hippocampus

Figure 5

Voltage-dye recordings demonstrate that PAR1 activation causes an increase in amplitude of NMDA receptor-mediated EPSP in dentate of hippocampus. A) Bright field image of mouse dentate hippocampus, loaded with the voltage-dye, di-4-ANEPPS. The boxed region is imaged during electrical stimulation with a stimulating electrode, as shown. Scale bar is 300 μm. B) The left panel is voltage-dye image during a synaptic response in the presence of 10 μM bicuculline, and 10 μM CNQX. Changes in intensity correspond to degree of voltage changes and are represented in pseudo-color. The scale bar is 50 μm. The traces (right panel) show the time course of intensity changes averaged from pixels in a selected region of interest over the cell body layer. The time courses for each condition are overlaid and plotted; all waveforms are corrected using a baseline slope from unstimulated records to account for time-dependent changes in voltage dye fluorescence. The experiment was performed from four different mice, respectively. C) The time course of normalized field EPSP amplitude in the presence of 2 μM CNQX. Black trace represents the thrombin treated slice. Blue trace represents control slice. Slices were from six mice (control) and eight mice (thrombin). D) Similar experiments are repeated and the results are summarized in this bar graph. Bar graphs show % increase over control of NMDA EPSP by voltage dye imaging or field recording. The average % increase is expressed as mean ± SEM (* p < 0.05).

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