Figure 3

Identification of neural crest-derived cells in the olfactory epithelium. Confocal images of the olfactory epithelium in Wnt1-Cre/Floxed-EGFP (a, b) and P0-Cre/Floxed-EGFP (c-e) mice stained for the indicated markers reveal the presence of neural crest-derived ICAM1⁺ HBCs, Mash1⁺ GBCs, and CK18⁺ SUS cells in the olfactory epithelium. All images of GFP are direct fluorescence observed in postnatal 4-week mice, except for the Mash1 staining in c that was observed in postnatal 2-day mice and required anti-GFP immunostaining after antigen retrieval procedures. OMP⁺ ORNs (f) and ICAM1⁺ HBCs (g) were observed in 4 week-old P0-Cre/Floxed-LacZ (f, g) mice. Nuclei were counterstained with Hoechst. Arrows in b and e indicate CK18⁺ ducts of Bowman's glands and asterisks indicate the nasal cavity. Scale bars: 50 μm.