Figure 3

Tracking lateral movements of single receptors. (A) Differential interference contrast imaging of a dendrite in neuronal culture. Simultaneous (B) FM1-43 labelling of presynaptic terminals and (C) single particle labelling of the GluR2 subunit of AMPARs by fluorescently-tagged antibodies allows receptor movements to be observed in synaptic and extrasynaptic space. Scale bar = 1 μm. (D) Sample receptor trajectories in synaptic (green) and extrasynaptic (red) space; (D1) Trajectory of fluorescently tagged antibodies fixed on a coverslip. Notice that receptor mobility within a synapse is generally more restricted than movement in extrasynaptic space (D2 & D3 vs.D4). (D5) Trajectory depicting the movement of an extrasynaptic receptor into the synapse. Figure 3: Reprinted by permission from Macmillan Publishers Ltd: [EMBO JOURNAL] (Tardin et al., 22(18):4656-65) copyright (2011).