hSnf7-1 and hSnf7-2 are expressed in human post-mitotic neurons. (A) qRT-PCR showed that hSnf7-2 was highly expressed in human neurons, while hSnf7-3 was barely detectable. (B) hESCs were immunostained with hSnf7-2. hSnf7-2 was mostly localized to cytoplasm. (C) Nucleus staining (indicated by DAPI staining) of hESCs as shown in panel B. (D) Immunostaining showed that hSnf7-1 was mostly expressed in the cytoplasm of human neurons. Bar: 20 mm. (E) Immunostaining with a hSnf7-2-specific antibody reveals hSnf7-2 in both the nucleus and cytoplasm of human neurons. Bar: 20 μm.