Both hSnf7-1 and hSnf7-2 are essential for the survival of human neurons. Terminally differentiated human neurons (14-16 DIV) were infected with lentivirus expressing either hSnf7-1 or hSnf7-2 siRNA. (A) Western blot analysis showed hSnf7-1 and hSnf7-2 specifically their expression. (B) Infection of lentivirus expressing either hSnf7-1 or hSnf7-2 siRNA caused human neuronal cell loss, respectively. GFP-positive and PI-negative neurons were considered as surviving neurons.