Differential regulation of diverse transmembrane cargos in human neurons by ESCRT-III with different subunit compositions. (A) Preferential interactions between hSnf7-1 and CHMP2A and between hSnf7-2 and CHMP2B. Flag-tagged CHMP2A or CHMP2B was transfected into HEK293T cells, and endogenous hSnf7-1 and hSnf7-2 proteins were detected by western blot with specific antibodies after IP with anti-Flag antibody. (B) siRNA knockdown of hSnf7-1 or hSnf7-2 in human neurons had differential effects on the accumulation of different transmembrane cargos. Human neurons were collected 3 days after infection with lentivirus expressing hSnf7-1 or hSnf7-2 siRNA. Actin was used as the loading control.