DIP/WISH protein expression in mouse brain. (A) Western blot analysis of DIP/WISH expression in several brain regions, heart, and lung of mice. (B) DIP/WISH mRNA expression in mouse hippocampus. (C) DIP/WISH protein expression in a dissociated mouse hippocampal neuron after 6 days in vitro (DIV, left) and 18 DIV (right). Scale bars indicate 500 μm (B), 50 μm (C, 6 DIV) and 10 μm (C, 18 DIV).