Figure 2

Galectin-1 is expressed in type 1 and 2a cells in the adult hippocampus. (A) Schematic diagram of neural marker expression in the adult DG, correlated with neuronal cell stage. DG, dentate gyrus; LV, lateral ventricle; Gr, granule layer; SGZ, subgranular zone (B-C) Three-dimensional reconstructions from confocal optical images of Galectin-1 (green) and GFAP (red) double-labeling, and Hoechst (blue) nuclear staining in the hippocampus. (B) Galectin-1+/GFAP+ cells were seen in the DG (arrow). (C) A projected image from one optical slice of B. (D-J) Galectin-1 expressed in type 1 and 2a cells in the adult DG. Arrows indicate FABP7 (red) and Galectin-1 (green) double-positive (yellow) cells (D), Nestin (red) and Galectin-1 (green) double-positive (yellow) cells (E), and Msi1 (red) and Galectin-1 (green) double-positive (yellow) cells (F) in the DG. (G) No co-labeling for Galectin-1+ (green; arrows) and DCX+ (red; arrowheads) in DG cells was seen. (H) Galectin-1+ (green; arrows) cells did not express Calretinin (red; arrowhead). (I) Galectin-1 (green; arrows) was not expressed in NeuN+ (red) cells. Note the presence of Galectin-1-positive interneurons in the hilus (arrowheads). (J) Galectin-1+ (green; arrowheads) cells did not express the blood vessel marker PE-CAM1 (red; arrows). DG, dentate gyrus; LV, lateral ventricle; Gr, granule layer; SGZ, subgranular zone. (Scale bars: B-C, 10 μm; D-J, 5 μm).