TRPM2 mRNA levels do not change in hippocampal primary cultures with time in vitro. (A) TRPM2 mRNA was measured at 14, 21, and 29 DIV (n = 8 per group) by quantitative real-time PCR (qPCR). No significant change in cycle threshold values is observed (p = 0.89) (B) QPCR analysis of neuron specific enolase (ENO2) mRNA values used to control for neuronal content over time. No significant difference was observed (p = 0.9). (C) Normalized TRPM2 values for each sample (ENO2-TRPM2) were quantified. No difference in the normalized levels of TRPM2 mRNA is seen over time in culture (p = 0.51).