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Figure 5 | Molecular Brain

Figure 5

From: Loss of glutathione homeostasis associated with neuronal senescence facilitates TRPM2 channel activation in cultured hippocampal pyramidal neurons

Figure 5

GSH inhibits TRPM2 in a thiol-independent mechanism. TRPM2 currents were elicited in HEK293-TRPM2 cells using a 2 mM calcium extracellular solution and 0.1 mM ADPR. TRPM2 currents were then inhibited and quantified following removal of extracellular calcium (A) Representative TRPM2 responses obtained in control (n = 12), GSH (n = 10), DTT (n = 6) and ophthalmic acid (n = 6) groups (B) TRPM2 currents are significantly inhibited with 10 mM GSH or 10 mM ophthalmic acid, but are not altered when 10 mM DTT is included in the patch pipette (One-way ANOVA followed by post-hoc Tukey's test, * p < 0.05).

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