Figure 3
From: Direct interaction between GluR2 and GAPDH regulates AMPAR-mediated excitotoxicity
Regulation of the AMPAR-mediated cell death in transfected cells. A, Bar graph summarizing the quantitative measurements of PI fluorescence from HEK-293 T cells expressing GluR1/2 subunits with/without glutamate treatment (300 μM glutamate, 25 μM CTZ, 24 hr). ***Significantly different from control group (P < 0.001, n = 9 per group), t-test. B, Bar graph summarizing the quantitative measurements of PI fluorescence from HEK-293 T cells expressing GluR1/2 subunits with/without glutamate treatment at various doses in the presence/absence of GluR2NT1-3–2 peptide (10 μM, 1 hr). **, *** Significantly different from control group (P < 0.01, 0.001), ANOVA followed by post-hoc SNK test; ##, significant from the corresponding glutamate group (P < 0.01, n = 9 per group), t-test. C, Bar graph summarizing the quantitative measurements of PI fluorescence from non-transfected HEK-293 T cells or HEK-293 T cells expressing GluR1/2 subunits. Cells were pre-treated with the GluR2NT1-3–2 peptide with/without glutamate treatment (n = 9 per group). D, Bar graph summarizing the quantitative measurements of PI fluorescence from HEK-293 T cells expressing GluR1/3, GluR1/4 or GluR3/4 subunits with glutamate treatment in the presence/absence of the GluR2NT1-3–2 peptide (n = 9 per group). All PI fluorescence measurement assays were performed 3 times independently.