Robust spinal neuroinflammation mediates mechanical allodynia in Walker 256 induced bone cancer rats

Table 1 Sequences of the forward and reverse primers and PCR conditions used for RT-PCR

GenBank accession	Target gene	Primers	PCR condition (temperature/time)			Predicted size (bp)
			Denature	Anneal	Extend
NM031144	β-actin	Forward: 5’-tcaggtcatcactatcggcaat-3’	94 °C/45 s	58 °C/45 s	72 °C/1 min	432
		Reverse: 5’-aaagaaagggtgtaaaacgca-3’
NM031512	IL-1β	Forward: 5’-atgagagcatccagcttcaaatc-3’	94 °C/45 s	58 °C/45 s	72 °C/1 min	328
		Reverse: 5’-gcttatgttctgtccattgaggt -3’
NM012589	IL-6	Forward: 5’-gacaaagccagagtccttca-3’	94 °C/45 s	58 °C/45 s	72 °C/1 min	229
		Reversed 5’-actaggtttgccgagtagac-3’
X66539	TNF-α	Forward: 5’-cgagatgtggaactggcaga-3’	94 °C/45 s	58 °C/45 s	72 °C/1 min	256
		Reverse: 5’-ctacgggcttgtcactcga-3’
NM019178	TLR4	Forward: 5’-gccggaaagttattgtggtg-3’	94 °C/45 s	58 °C/45 s	72 °C/1 min	203
		Reverse: 5’-ccactcgaggtaggtgttt-3’

ISSN: 1756-6606