Generation of astrocytes from iPSCs. A: A multi-step protocol was used to generate astrocytes. iPSCs were initially differentiated into neurospheres using a feeder-free protocol and subsequently differentiated into astrocytes. B: Astrocytes generated from HD-iPSCs exhibited a typical astrocyte morphology and expressed astrocyte markers glial fibrillary acidic protein (GFAP) and S100β. Scale bars: 100 μm (A) or 20 μm (B).