Skip to main content
Figure 2 | Molecular Brain

Figure 2

From: mGluR1,5 activation improves network asynchrony and GABAergic synapse attenuation in the amygdala: implication for anxiety-like behavior in DBA/2 mice

Figure 2

The activity strength of amygdala neurons is not different in DBA/2 and FVB/N mice. Oregon -green BAPTA-AM was loaded into the cells in brain slices including amygdala to monitor Ca2+ levels in neurons and astrocytes. Sulforhodanmine-101 was used to label astrocytes. Fluorescents in amygdala areas were excited and detected by two-photon laser scanning microscopy. A) shows a photo of Ca2+ imaging from the neurons (green) and astrocytes (red and yellow) in DBA/2 mice. B) shows Ca2+ imaging from the neurons (green) and astrocytes (red/yellow) in FVB/N mice. C) shows the number of neurons vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). D) shows the number of spontaneous events from neurons versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve). E) shows the number of astrocytes vs. their absolute fluorescence intensity (AFI) in DBA/2 mice (gray bars/fitting curve; n = 12 for mice and n = 27 for slices) and FVB/N mice (white bars/black curve; n = 11 for mice and n = 25 for slices). F) shows the number of spontaneous events from astrocytes versus their relative fluorescence intensity (ΔF/F0) in DBA/2 (gray bars/fitting curve) and FVB/N mice (white bars/black curve).

Back to article page