Figure 8
The effect of 3,5-DHPG on the synchronous activity of amygdala neurons in DBA/2 mice. Oregon-green BAPTA-AM was loaded into the cells in brain slices including amygdala to monitor Ca2+ levels in neurons. OGB-1 in amygdala were excited and detected by a two-photon laser scanning microscopy before and after DHPG (10 μM) was washed onto the slices. A ~ B) The pictures of chip patterns show the cross-correlations in the timing phase of activity between neighboring neurons under the conditions of control (A) and 3,5-DHPG wash-on (B). Color red presents a high cross-correlation (synchronous activity). C) shows a comparison in correlation coefficients averaged from all of the visible amygdala neurons before (black symbols/line) and after (red) washing 3,5-DHPG to a slice from a DBA/2 mouse (p < 0.01). D) illustrates correlation coefficients averaged from all of experimental DBA/2 mice (n = 7) before (black symbols and line) and after (red) washing-on DHPG (p < 0.01). E) shows the comparison in the number of cells vs. their absolute fluorescence intensity (AFI) before (gray bars/fitting curve) and after applying DHPG (white bars/black curve) in DBA/2 mice. F) shows a comparison in the number of spontaneous events versus their relative fluorescence intensity (ΔF/F) before (gray bars/fitting curve) and after applying DHPG (white bars/black curve) in DBA/2 mice.