The change in expression of NICD in DRG neurons in control and CCI model rats. (A) Immunostaining showing the expression of NICD in the DRG. NICD was present in small neurons in the DRG, and nerve injury did not induce significant change. Scale bar, 100 μm. (B) RT-PCR revealed Notch1 expression in DRG tissue, while nerve injury did not induce a significant change. (C) Immunoblotting showed that in the rat DRG, the levels of NICD did not markedly change after nerve injury (CCI model) (n = 3). The data represents three independent experiments. (D) Immunostaining revealed that NICD co-localized with substance P, but did not co-localize with IB4 in the DRG. Scale bar, 100 μm.